Protocol for production and purification of SARS-CoV-2 3CLpro
Description
3CL^(pro) protease from SARS-CoV-2 is a primary target for COVID-19 antiviral drug development. Here, we present a protocol for 3CL^(pro) production in Escherichia coli. We describe steps to purify 3CL^(pro), expressed as a fusion with the
